Composite

Part:BBa_K2967028:Design

Designed by: Shuang Wu   Group: iGEM19_NEU_CHINA   (2019-10-06)


Expressing vector for YebF-Myrosinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 689
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 918
    Illegal BsaI site found at 1947


Design Notes

We need myrosinase to mediate the conversion of glucosinolate to sulforaphane. So we added a YebF secretory tag to secret this protein.


Source

The myrosinase gene comes from cruciferous vegetable.

References